1A’an Haries Pranowo, 2Danis Pertiwi, 2Agung Putra
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2Stem Cell and Cancer Research, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v6-i10-44Google Scholar Download Pdf
ABSTRACT:
The autoreactivity of T cells in DMT1 patients causes activation of M1 and M2 macrophages as proinflammatory and anti-inflammatory markers which promote pancreatic cell damage. Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) therapy methods play a role in repairing damaged tissue and regenerating pancreatic islet cells. This study aims to examine the effect of SH-MSCs on macrophage cell polarization through the expression of CD68 as a marker of M1 macrophages and CD163 as a marker of M2 macrophages in DMT1 rats. The sample of this study were 5 Wistar rats which were divided into 4 groups; normal group (no treatment), negative control group, and 2 treatment groups with 0.5cc of SH-MSCs (P1 group) and 1cc of SH-MSCs (P2 group). STZ 60 mg/kgBW induced DMT1 in the negative control, P1, and P2 groups. Data were tested parametrically using SPSS 22. The mean of CD68 expression in normal group was 3.06±0.35%, negative control: 21.67±0.93%, P1: 10.98±0.68%, P2: 5.85±0.55%. The mean of CD163 expression in normal group: 22.83±0.51%, negative control group: 3.56±0.50%, P1:15.81±1.80%, P2: 20.80±2.24%. The results of the normality and homogeneity test were p> 0.05. Anova test results p < 0.05. Post Hoc LSD test results p < 0.05. The administration of SH-MSCS was shown to decrease CD68 and increase the CD163 expression significantly in DMT1 rats.
KEYWORD:CD68, CD163, M1 macrophages, M2 macrophages, DMT1
REFERENCES1) Internasional Diabetes Federation (IDF), Diabetes Atlas edition 10th,2021.(n.d.); www.diabetesatlas.org
2) S,Pulungan.,dkk. Diabetes melitus tipe-1 pada anak: situasi di Indonesia dan tata laksana Diabetes Melitus Tipe-1 pada Anak. Intisari Sari Pediatri (Vol. 20, Issue 6).2019.
3) Mathew P, Thoppil D. Hypoglycemia Treasure Island (FL). 2021 https://www.ncbi.nlm.nih.gov/books/NBK534841/
4) Mukhtar, D. KEDOKTERAN MAKROFAG PADA JARINGAN ADIPOSA OBES SEBAGAI PENANDA TERJADINYA RESISTENSI INSULIN. Yarsi Univercity. Jakarta. 2012.
5) Putra, A., Hadi Suwiryo, Z., Muhar, A. M., Widyatmoko, A., & Rahmi, F. L. (n.d.). The Role of Mesenchymal Stem Cells in Regulating PDGF and VEGF during Pancreatic Islet Cells Regeneration in Diabetic Animal Model. Folia Medica. 2020. 63(6), 875–883. https://doi.org/10.3897/folmed.63.e57636
6) Yustianingsih V, Sumarawati T, Putra A. Hypoxia enhances self-renewal properties and markers of mesenchymal stem cells. Universa Med 2019; 38: 164.
7) IKATAN DOKTER ANAK INDONESIA (IDAI). Praktik Klinis, Diagnosis dan Tata Laksana Diabetes Melitus Tipe-1 pada Anak dan Remaja.2017.
8) Fitria Nusantara, A.,Hafshawaty. SUPPORT SYSTEM KELUARGA DALAM PENCEGAHAN KETOASIDOSIS DIABETIK PADA ANAK DENGAN DM TIPE 1. Jurnal Ilmu Kesehatan, 3(1).2019.
9) Johannesson, B., Sui, L., Freytes, D. O., Creusot, R. J. Toward beta cell replacement for diabetes. The EMBO journal,34(7).2015 841–855. https://doi.org/10.15252/embj.201490685
10) Virana Putri, N., S.P.,Rasyid, R., Murti, K. Relationship between Rumor Associated Macrophages and Tumor Associated Neutrophils Density with Clinicihistopathological Characterictics of Hodgkin`s Lymphoma Patients. Histologi and Anatomic Pathologi Departement of Sriwijaya Univercity. 2021. (Vol. 30, Issue 2).
11) Bosco, M. C. Macrophage polarization: Reaching across the aisle? In Journal of Allergy and Clinical Immunology. 2019. (Vol. 143, Issue 4, pp. 1348–1350). Mosby Inc. https://doi.org/10.1016/j.jaci.2018.12.995
12) Castellanos, dkk. Effectiveness of mesenchymal stem cell-conditioned medium in bone regeneration in animal and human models: a systematic review and meta-analysis. 2020; 9: 1–22.
13) M. Madrigal., KS, Rao., NH., Riorden. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods. J Transl Med 2014; 12: 1–14
14) Robert, A.W., Marcon, B.H., Dallagiovanna, B. and Shigunov. Adipogenesis, osteogenesis, and chondrogenesis of human mesenchymal stem/stromal cells: a comparative transcriptome approach. Frontiers in Cell and Developmental Biology. 2020;8:561.
15) Caroti, C.M., Ahn, H., Salazar, H.F., Joseph, G., Sankar, S.B., Willett, N.J., Wood, L.B., Taylor, W.R. and Lyle, A.N. A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained. NIH, 2017. doi: 10.1038/s41598-017-13477-y
16) J.Lucier & R.S.Weinstock. Diabetes Mellitus Type 1, PubMed, 2022 https://pubmed.ncbi.nlm.nih.gov/29939535/
17) Nyoman.,W. Peningkatan produks radikal bebas pada kasus diabetes melitus, Jurnal FK Udayana , Bali. 2015
18) Wang Y, Branicky R, Noë A, Hekimi,S. Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J Cell Biol 2018; 217. DOI: 10.1083/jcb.201708007
19) Kar S, Subbaram S, Carrico PM, Melendez JA. Redox-control of matrix metalloproteinase-1: A critical link between free radicals, matrix remodeling and degenerative disease. Respir Physiol Neurobiol 2010; 174: 299–306.
20) Tran C, Damaser MS. Stem cells as drug delivery methods: Application of stem cell secretome for regeneration. Adv Drug Deliv Rev. 2015;82:1–11.
21) Xiao Y.,Li X., Hao H., Cui Y., Chen M, Liu L, et,al. Secretome of Mesencymal stem cell. China. 2013.
22) Putra A, Pertiwi D, Milla MN, Indrayani UD, Jannah D, Sahariyani M et al. Hypoxia-preconditioned MSCs have superior effect in ameliorating renal function on acute renal failure animal model. Open Access Maced J Med Sci 2019; 7: 305–310.
23) Chistiakov, D. A., Killingsworth, M. C., Myasoedova, V. A., Orekhov, A. N., & Bobryshev, Y. CD68/macrosialin: Not just a histochemical marker. Laboratory Investigation. 2017; 97(1), 4–13. https://doi.org/10.1038/labinvest.2016.116
24) Hovsepian, E., Penas, F., Siffo, S., Mirkin, G. A., & Goren, N. B. IL-10 inhibits the NF-κB and ERK/MAPK-mediated production of pro-inflammatory mediators by up-regulation of SOCS-3 in Trypanosoma cruzi-infected cardiomyocytes. PLoS ONE, 8(11). 2013. https://doi.org/10.1371/journal.pone.0079445
25) Lang, R., Rutschman, R. L., Greaves, D. R., & Murray, P. J . Autocrine Deactivation of Macrophages in Transgenic Mice Constitutively Overexpressing IL-10 Under Control of the Human CD68 Promoter. The Journal of Immunology. 2002; 168(7), 3402–3411. https://doi.org/10.4049/jimmunol.168.7.3402
26) Orecchioni, M., Ghosheh, Y., Pramod, A. B., & Ley, K. Macrophage polarization: Different gene signatures in M1(Lps+) vs. Classically and M2(LPS-) vs. Alternatively activated macrophages. In Frontiers in Immunology (Vol. 10, Issue MAY). Frontiers Media S.A. 2019. https://doi.org/10.3389/fimmu.2019.01084
27) Wang, J., Xia, J., Huang, R., Hu, Y., Fan, J., Shu, Q., & Xu, J. Mesenchymal stem cell-derived extracellular vesicles alter disease outcomes via endorsement of macrophage polarization. In Stem Cell Research and Therapy (Vol. 11, Issue 1). BioMed Central Ltd. 2020. https://doi.org/10.1186/s13287-020-01937-8
28) Jamiyan, T., Kuroda, H., Yamaguchi, R., Abe, A., & Hayashi, M. (n.d.). CD68- and CD163-positive tumor-associated macrophages in triple negative cancer of the breast. 2020. https://doi.org/10.1007/s00428-020-02855-z/Published
Volume 06 Issue 10 October 2023
There is an Open Access article, distributed under the term of the Creative Commons Attribution – Non Commercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/), which permits remixing, adapting and building upon the work for non-commercial use, provided the original work is properly cited.
Our Services and Policies
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected.
The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.
International Journal of Multidisciplinary Research and Analysis will publish 12 monthly online issues per year,IJMRA publishes articles as soon as the final copy-edited version is approved. IJMRA publishes articles and review papers of all subjects area.
Open access is a mechanism by which research outputs are distributed online, Hybrid open access journals, contain a mixture of open access articles and closed access articles.
International Journal of Multidisciplinary Research and Analysis initiate a call for research paper for Volume 07 Issue 12 (December 2024).
PUBLICATION DATES:
1) Last Date of Submission : 26 December 2024 .
2) Article published within a week.
3) Submit Article : editor@ijmra.in or Online
Why with us
1 : IJMRA only accepts original and high quality research and technical papers.
2 : Paper will publish immediately in current issue after registration.
3 : Authors can download their full papers at any time with digital certificate.
The Editors reserve the right to reject papers without sending them out for review.
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected. The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.